NBT/BCIP Ready-to-Use Tablets NBT/BCIP -20 TAB
NBT/BCIP Ready-to-Use Tablets
HSN : 38220090
Quality Level 100
form tablet
mol wt Mr 433.6 (BCIP toluidine salt)
Mr 817.7 (NBT)
packaging pkg of 20 tablets
shipped in wet ice
storage temp. 2-8°C
General description
BCIP is the alkaline phosphatase (AP) substrate, which after dephosphorylation is oxidized by NBT to yield a dark-blue indigo precipitating dye.[3] NBT is thereby reduced to a dark-blue precipitating dye and serves to intensify the color reaction making the detection more sensitive.
The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.
Application
NBT/BCIP Ready-to-Use tablets has been used in western blotting [4] and in situ hybridization.[5]
Preparation Note
Working solution: Preparation of Color Substrate Solution
Add 1 tablet to 10 ml double-dist. water to prepare a ready-to-use staining solution.
Composition of staining solution (pH 9.5) after addition of double-dist. water: 0.4 mg/ml NBT; 0.19 mg/ml BCIP; 100 mM Tris buffer, pH 9.5; 50 mM MgSO4
Information Note: The color of the solution can vary from slightly yellowish-violet to light brown.
Important Note: Prepare the solution shortly before use.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Description
NBT/BCIP Ready-to-Use Tablets
HSN : 38220090
Quality Level 100
form tablet
mol wt Mr 433.6 (BCIP toluidine salt)
Mr 817.7 (NBT)
packaging pkg of 20 tablets
shipped in wet ice
storage temp. 2-8°C
General description
BCIP is the alkaline phosphatase (AP) substrate, which after dephosphorylation is oxidized by NBT to yield a dark-blue indigo precipitating dye.[3] NBT is thereby reduced to a dark-blue precipitating dye and serves to intensify the color reaction making the detection more sensitive.
The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.
Application
NBT/BCIP Ready-to-Use tablets has been used in western blotting [4] and in situ hybridization.[5]
Preparation Note
Working solution: Preparation of Color Substrate Solution
Add 1 tablet to 10 ml double-dist. water to prepare a ready-to-use staining solution.
Composition of staining solution (pH 9.5) after addition of double-dist. water: 0.4 mg/ml NBT; 0.19 mg/ml BCIP; 100 mM Tris buffer, pH 9.5; 50 mM MgSO4
Information Note: The color of the solution can vary from slightly yellowish-violet to light brown.
Important Note: Prepare the solution shortly before use.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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