CFG PR 5641 50ml Cell freezing Medium Glycerol with FBS & Glycerol w/o Antibiotic (sterile filtered)
€490.00
Cell freezing Medium Glycerol with FBS & Glycerol w/o Antibiotic (sterile filtered)
Product No CFG PR 5641 Pack. sz. 50ML
CFG PR 5641 50ml
For R & D use only , Not for Drug or other uses.
Storage : -20*c
Cell Freezing Media are complete, ready to
use reagents designed to protect and preserve cells during
frozen storage. These media are a convenient and cost
effective alternative to in-house freezing media and can
be used for a wide variety of mammalian cells.
Cell Freezing Medium with Glycerol is fully
supplemented formulation prepared in Dulbecco’s
Modified Eagle Medium. It contains 10% Glycerol and
foetal bovine serum. Glycerol acts a cryoprotectant as
it prevents formation of ice crystals and prevents cell
damage. It is ready to use and does not require further
addition of any other reagents.
This cryopreservation medium can be used for hardy cell
lines that are less susceptible to freezing damage. Users
are advised to test the suitability of the medium for sensitive
Cell lines
Composition :
Ingredients % (V/V)
OTHERS
Dulbecco’s Modified Eagle Medium Made to volume
Fetal bovine serum Proprietary
Glycerol 10%
Directions :
Cell freezing medium can be used with standard freezing
protocols. The following protocol may be used.
Thaw cell culture freezing medium, mix well, and keep on
wet ice during use.
Procedure for freezing:
1. For optimum results, cells should be in log phase of
growth.
2. Gently detach adherent cells from the surface using
Trypsin or other appropriate means.
3. Gently pellet the cell suspension by centrifugation (200
to 400 x g for 5 minutes for suspension cells and 200 x g
for 5 minutes for adherent cells). Using a pipette, remove
the medium above the pellet down to the smallest volume
without disturbing the cells.
4. Resuspend the cells in Cell Freezing Medium at a
recommended density for a specific cell type. Hybridoma
cells may require higher cell density.
5. Aliquot cells in appropriate cryogenic storage vials. Freeze
the cells in a controlled rate freezing apparatus, decreasing
the temperature approximately 1ºC per minute. Alternatively,
place the cryovials containing the cells in an isopropanol
chamber and store them at -80ºC overnight. Alternatively,
store them at -20ºC for 1 – 2 hours before shifting to -80ºC
overnight.
6. Transfer cryovials to liquid nitrogen tank for long term
storage.
Procedure for thawing of cryopreserved cells:
1. Remove cells from frozen storage and quickly thaw in a
37ºC water bath.
2. Dilute 1ml suspension with 10ml of complete growth
medium.
3. Mix cells gently and pellet by gentle centrifugation.
4. Discard the supernatant and gently resuspend the cells
in complete growth medium and seed in appropriate culture
vessel.
1. Cells harvested for cryopreservation should be at their
optimum viability to ensure maximum survival during
freezing and after thawing.
2. On removal from storage, extreme caution must be
exercised to prevent explosion of the cryovial because of
sudden expansion of the trapped nitrogen.
3. To retain maximum viability during cryopreservation,
cells must be cooled at a constant slow rate, -1 to -5ºC/min.
This can be achieved using programmable freezers or placing
ampoules in a heavily insulated box at -80ºC for 24 hours
before transferring them to their final storage location.
4. After thawing cells, it is necessary to slowly dilute the
croprotectant to prevent osmotic shock. When it is necessary
to centrifuge the cells, use the minimum g force to sediment
them to prevent shearing damage, i.e. 70-100g.
5. To initiate rapid growth, it is advisable to inoculate new
cultures at a higher density than for routine subculture, e.g.,
between 3 and 4 X 104
viable cells/cm2
for adherent cells.
6. The minimum number of tests that should be carried out
on master cell banks are, total and viable cell counts, growth
potential, screening for bacteria, fungi and mycoplasma and
cell line authenticity.
Quality Control:
Appearance
Red colored clear solution.
pH 7.60 -8.20
Osmolality in mOsm/Kg H2O 1800.00 -2200.00
Sterility No bacterial or fungal growth is observed after 14 days of
incubation, as per USP specification. Performance test
Performance test is done by freezing cells and doing a
viability assessment after thawing and comparing it with a
control medium.
Description
Cell freezing Medium Glycerol with FBS & Glycerol w/o Antibiotic (sterile filtered)
Product No CFG PR 5641 Pack. sz. 50ML
CFG PR 5641 50ml
For R & D use only , Not for Drug or other uses.
Storage : -20*c
Cell Freezing Media are complete, ready to
use reagents designed to protect and preserve cells during
frozen storage. These media are a convenient and cost
effective alternative to in-house freezing media and can
be used for a wide variety of mammalian cells.
Cell Freezing Medium with Glycerol is fully
supplemented formulation prepared in Dulbecco’s
Modified Eagle Medium. It contains 10% Glycerol and
foetal bovine serum. Glycerol acts a cryoprotectant as
it prevents formation of ice crystals and prevents cell
damage. It is ready to use and does not require further
addition of any other reagents.
This cryopreservation medium can be used for hardy cell
lines that are less susceptible to freezing damage. Users
are advised to test the suitability of the medium for sensitive
Cell lines
Composition :
Ingredients % (V/V)
OTHERS
Dulbecco’s Modified Eagle Medium Made to volume
Fetal bovine serum Proprietary
Glycerol 10%
Directions :
Cell freezing medium can be used with standard freezing
protocols. The following protocol may be used.
Thaw cell culture freezing medium, mix well, and keep on
wet ice during use.
Procedure for freezing:
1. For optimum results, cells should be in log phase of
growth.
2. Gently detach adherent cells from the surface using
Trypsin or other appropriate means.
3. Gently pellet the cell suspension by centrifugation (200
to 400 x g for 5 minutes for suspension cells and 200 x g
for 5 minutes for adherent cells). Using a pipette, remove
the medium above the pellet down to the smallest volume
without disturbing the cells.
4. Resuspend the cells in Cell Freezing Medium at a
recommended density for a specific cell type. Hybridoma
cells may require higher cell density.
5. Aliquot cells in appropriate cryogenic storage vials. Freeze
the cells in a controlled rate freezing apparatus, decreasing
the temperature approximately 1ºC per minute. Alternatively,
place the cryovials containing the cells in an isopropanol
chamber and store them at -80ºC overnight. Alternatively,
store them at -20ºC for 1 – 2 hours before shifting to -80ºC
overnight.
6. Transfer cryovials to liquid nitrogen tank for long term
storage.
Procedure for thawing of cryopreserved cells:
1. Remove cells from frozen storage and quickly thaw in a
37ºC water bath.
2. Dilute 1ml suspension with 10ml of complete growth
medium.
3. Mix cells gently and pellet by gentle centrifugation.
4. Discard the supernatant and gently resuspend the cells
in complete growth medium and seed in appropriate culture
vessel.
1. Cells harvested for cryopreservation should be at their
optimum viability to ensure maximum survival during
freezing and after thawing.
2. On removal from storage, extreme caution must be
exercised to prevent explosion of the cryovial because of
sudden expansion of the trapped nitrogen.
3. To retain maximum viability during cryopreservation,
cells must be cooled at a constant slow rate, -1 to -5ºC/min.
This can be achieved using programmable freezers or placing
ampoules in a heavily insulated box at -80ºC for 24 hours
before transferring them to their final storage location.
4. After thawing cells, it is necessary to slowly dilute the
croprotectant to prevent osmotic shock. When it is necessary
to centrifuge the cells, use the minimum g force to sediment
them to prevent shearing damage, i.e. 70-100g.
5. To initiate rapid growth, it is advisable to inoculate new
cultures at a higher density than for routine subculture, e.g.,
between 3 and 4 X 104
viable cells/cm2
for adherent cells.
6. The minimum number of tests that should be carried out
on master cell banks are, total and viable cell counts, growth
potential, screening for bacteria, fungi and mycoplasma and
cell line authenticity.
Quality Control:
Appearance
Red colored clear solution.
pH 7.60 -8.20
Osmolality in mOsm/Kg H2O 1800.00 -2200.00
Sterility No bacterial or fungal growth is observed after 14 days of
incubation, as per USP specification. Performance test
Performance test is done by freezing cells and doing a
viability assessment after thawing and comparing it with a
control medium.
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